Rapid serological diagnosis of enterovirus 71 infection by IgM ELISA.

نویسندگان

  • Yoshio Tano
  • Hiroyuki Shimizu
  • Masashi Shiomi
  • Takashi Nakano
  • Tatsuo Miyamura
چکیده

Yoshio Tano, Hiroyuki Shimizu*, Masashi Shiomi, Takashi Nakano and Tatsuo Miyamura Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashimurayama, Tokyo 208-0011, Japan Poliomyelitis Research Institute, Kumegawa-cho 5-34-4, Higashimurayama-shi, Tokyo 189-0003, Osaka City General Hospital, Miyakojima-hondori 1-13-22, Miyakojima-ku, Osaka 534-0021 and Mie National Hospital, Osatokubota-cho 357, Tsu-shi, Mie 514-0125

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Performance of Detecting IgM Antibodies against Enterovirus 71 for Early Diagnosis

Enterovirus 71 (EV71) infection is more likely to induce severe complications and mortality than other enteroviruses. Methods for detection of IgM antibody against EV71 had been established for years, however, the performance of the methods in the very early diagnosis of EV71 infection had not been fully evaluated, which is especially meaningful because of the short incubation period of EV71 in...

متن کامل

Cytopathic effects of Campylobacter pylori urease.

I McCartney RA, Banatvala JE, Bell EJ. Routine use of p-antibody-capture ELISA for the serological diagnosis of Coxsackie B virus infections. J Med Virol 1986;19:205-12. 2 Pugh SF. Heterotypic reactions in a radioimmunoassay for Coxsackie B virus specific IgM. J Clin Pathol 1984;37:433-9. 3 Bell EJ, McCartney RA, Basquill D, Chaudhuri AKR. p-Antibody capture ELISA for the rapid diagnosis of ent...

متن کامل

Diagnosis of enterovirus infection by genus-specific PCR and enzyme-linked immunosorbent assays.

PCR for the diagnosis of enterovirus infections is resource intensive but is increasingly used due to wide availability. Enzyme-linked immunosorbent assays (ELISAs) that detect heterotypical antibodies against enterovirus immunoglobulin M (IgM), IgA, and IgG were compared with reverse transcription-PCR by using primers specific to the 5' untranslated regions of 60 enterovirus species. The ELISA...

متن کامل

Expression of enterovirus 71 capsid protein VP1 in Escherichia coli and its clinical application

The VPl gene of enterovirus 71 (EV71) was synthesized, construct a recombinant plasmid pET15b/VP1 and expressed in E. coli BL21. The recombinant VP1 protein could specifically react with EV71-infected patient sera without the cross-reaction with serum antibodies of coxsackievirus A16 (CA16), A4, A5, B3 and B5 as well as echovirus 6. In acute and convalescent phases, IgM and IgG antibodies of 18...

متن کامل

Serological and molecular diagnosis of human brucellosis in Najran, Southwestern Saudi Arabia.

This study aimed to investigate the prevalence of human brucellosis in Najran, southwestern Saudi Arabia, and to assess the performances of ELISA and PCR as diagnostic tools for brucellosis with respect to conventional methods. The study included 340 patients with clinical characteristics of brucellosis. Blood samples from cases and controls were subjected to culture, standard tube agglutinatio...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Japanese journal of infectious diseases

دوره 55 4  شماره 

صفحات  -

تاریخ انتشار 2002